Cloning symbiosis‐related cDNAs from eucalypt ectomycorrhiza by PCR‐assisted differential screening
نویسندگان
چکیده
منابع مشابه
Cloning of cDNAs encoding SODs from lettuce plants which show differential regulation by arbuscular mycorrhizal symbiosis and by drought stress.
In the present study three cDNA fragments were cloned using degenerate primers for Mn-sod genes and PCR: two showed a high degree of identity with Mn-sods from plants and the third with Fe-sod. Arbuscular mycorrhizal (AM) symbiosis down-regulated their expression pattern under well-watered conditions. In contrast, AM symbiosis in combination with drought stress considerably increased the expres...
متن کاملCloning CYP2D21 and CYP3A22 cDNAs from liver of miniature pigs.
To compare the identity of the primary structure of drug-metabolizing cytochrome P450 between miniature pigs and humans, two cDNA clones, coding for miniature pig CYP2D21 and CYP3A22, were isolated. The deduced amino acid sequences of CYP2D21 and CYP3A22 were 78.3 and 75.0% identical to human CYP2D6 and CYP3A4, respectively. These values were nearly the same as those of bovine, dog, and some ro...
متن کاملPractical method for cloning cDNAs generated in an mRNA differential display.
assembly with dimensions of 14 x 17 in. can accommodate 12 high-density arrayed filters (8 x 12 cm) or 15 circular filters (82 mm diameter). The overall results produce a significant increase in the throughput of the number of filters that can be processed with consistent results and the absence of any cross contamination. Cleaning the netting assembly is easily accomplished by rinsing with wat...
متن کاملMaize cytokinin oxidase genes: differential expression and cloning of two new cDNAs.
Cytokinin oxidases (CKOs) play a major role in the regulation of hormone levels in plants by irreversibly degrading cytokinins. Two new cDNAs from maize (CKO2 and CKO3) were cloned and CKO activity of a recombinant CKO3 enzyme was demonstrated. CKO2 and CKO3 encode flavoproteins with 93% identity among each other compared with 45% identity with CKO1. The respective genes were mapped to BIN 3.05...
متن کاملMolecular cloning of peroxidase cDNAs from dehydration-treated fibrous roots of sweetpotato and their differential expression in response to stress.
Three peroxidase (POD) cDNAs were isolated from dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas) plant via the screening of a cDNA library, and their expressions were assessed to characterize functions of each POD in relation to environmental stress. Three PODs were divided into two groups, designated the basic PODs (swpb4, swpb5) and the anionic PODs (swpa7), on the basis of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: New Phytologist
سال: 1993
ISSN: 0028-646X,1469-8137
DOI: 10.1111/j.1469-8137.1993.tb03884.x